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Figure 3


Fig. 3. High power images of differentiating myoblast cultures after 36 hours of treatment, co-stained for NM2A, actin and nuclei. In all the control cultures, cells are present that have a characteristic bipolar shape (arrows), stain strongly for actin compared with non-differentiated cells, and are beginning to align and fuse. By contrast, in NM2A-knockdown cultures, cells have an irregular shape (arrows), and do not form groups of aligned bipolar cells. Bar, 20 µm.





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