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Fig. 7. Immunofluorescent images of NM2B-knockdown cells after 72 hours of treatment in dense cultures shows that cells can still fuse. Cells were co-stained for skeletal myosin (red), actin (green) and DNA (blue). To the left are low-power images that show the presence of myotubes positive for skeletal myosin. To the right are high-power images each showing a myotube formed by NM2B-knockdown cells, compared with a myotube formed in control conditions (sense NM2B-treated). Five nuclei seen in the NM2B-knockdown cells are all very close together, whereas the two nuclei in a similar length of myotube formed by control cells are widely spaced.