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Fig. 8. Still images from a time-lapse experiment for NM2A- or NM2B-knockdown. Three sets of montages are shown, one each for untreated, NM2A- or NM2B-knockdown cells, from a single time-lapse DIC imaging experiment. The cells were left untreated or treated with antisense oligonucleotides for 24 hours before the start of filming, and filmed for the next 18 hours immediately after adding additional oligonucleotides. The numbers shown in the corner of each frame are the time elapsed since the first frame (time=0) in minutes (frame interval was 5 minutes). The arrows for the untreated cells show the progress of a single cell as it forms part of an aligned group of bipolar cells. The arrows for the NM2A-knockdown experiment show a single cell that tends to have a longer tail than controls but is able to retract. The arrows for the NM2B-knockdown experiment show a cell that becomes highly extended as it interacts with, and fails to detach from, other cells in the culture, and eventually its tail breaks (at t=185 minutes). See also supplementary material Movies 1-3.
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