JCS03066 Supplementary Material

Files in this Data Supplement:

• Movie 1A -

  Movie 1a. Alexa 568 transferrin in control cells approximately 5 minutes after transferrin internalization was induced by shift from 0°C to 37°C. Elapsed time is indicated (Minutes:Seconds).

• Movie 1B -

  Movie 1b. Alexa 568 transferrin (red) in control cells approximately 30 minutes after transferrin internalization was induced by shift from 0°C to 37°C. Frame size is approximately 65×70 μm. Elapsed time is indicated (Minutes:Seconds).

• Movie 2A -

  Movie 2a. Alexa 568 transferrin (red) in PtK1 cells stably expressing ^GFPRab11b-GDP (green), approximately 5 minutes after transferrin internalization was induced by shift from 0°C to 37°C. Frame size is approximately 65×70 μm. Elapsed time is indicated (Minutes:Seconds).

• Movie 2B -

  Movie 2b. Alexa 568 transferrin (red) in PtK1 cells stably expressing ^GFPRab11b-GDP (green), approximately 30 minutes after transferrin internalization was induced by shift from 0°C to 37°C. Frame size is approximately 65x70 μm. Elapsed time is indicated (Minutes:Seconds).

• Movie 3A -

  Movie 3a. Alexa 568 transferrin (red) in PtK1 cells stably expressing ^GFPRab11b-GTP (green), approximately 5 minutes after transferrin internalization was induced by shift from 0°C to 37°C. Frame size is approximately 65×70 μm. Elapsed time is indicated (Minutes:Seconds).

• Movie 3B -

  Movie 3b. Alexa 568 transferrin (red) in PtK1 cells stably expressing ^GFPRab11b-GTP (green), approximately 30 minutes after transferrin internalization was induced by shift from 0°C to 37°C. Frame size is approximately 70×70 μm. Elapsed time is indicated (Minutes:Seconds).

• Movie 4 -

  Movie 4. Migration of a control PtK1 island. Bar, 50 μm. Elapsed time is indicated (Minutes:Seconds).

• Movie 5 -

  Movie 5. Migration of PtK1 island where some of the cells were microinjected with ^GFPRab11b-GTP (black arrows in the first frame). Bar, 50 μm. Elapsed time is indicated (Minutes:Seconds).

• Movie 6 -

  Movie 6. Migration of PtK1 island where some of the cells were microinjected with ^GFPRab11b-GDP (white arrows in the first frame). Bar, 50 μm. Elapsed time is indicated (Minutes:Seconds).

• Movie 7 -

  Movie 7. Migration of a control PtK1 cells in a wound healing assay. Bar, 50 μm. Time is indicated (Month Date Hours:Minutes).

• Movie 8 -

  Movie 8. Migration of PtK1 cells, stably expressing ^GFPRab11b-GDPin a wound healing assay. Bar, 50 μm. Time is indicated (Month Date Hours:Minutes).

• Movie 9 -

  Movie 9. Migration of PtK1 cells, stably expressing ^GFPRab11b-GTPin a wound healing assay. Bar, 50 μm. Time is indicated (Month Date Hours:Minutes).