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Files in this Data Supplement:
Movie 1. Fluorescent time-lapse movie showing in situ images of leading protrusions elongating form cerebellar rhombic lip-derived neuronal precursors expressing EGFP. These leading protrusions are extending toward the ventral region (to the right in this movie). Actual duration of the movie is 120 minutes, and composed of 61 frames with 2 minutes intervals; Fig. 1F is made from the images of time points 0, 40, 80, 120 minutes. The same settings are used for making all the following movies and derived figures. However, the image regions of shown movies are trimmed to decrease their size.
Movie 2. Cell soma translocation of cerebellar rhombic lip-derived neuronal precursors visualized with EGFP.
Movie 3. Morphological changes in N17-Cdc42-expressing cells visualized with co-expressed EGFP. N17-Cdc42 abrogated the monopolar shape of neuronal precursors, and they show either a bipolar shape round morphology. The montage shown in Fig. 3A is derived from this movie.
Movie 4. Dynamics of leading protrusions on Par6-NT-expressing neuronal precursors visualized with co-expressed EGFP. These cells frequently show severe branching of their leading protrusion. Fig. 3B is made from this movie.
Movie 5. Dynamics of EGFP-expressing neuronal precursors treated with the microtubule depolymerizing drug, 5 μM nocodazole, for 15 minutes. These cells show fragmentation of their leading process. Fig. 3E originated from this movie.
Movie 6. Neuronal precursors visualized by expressing a microtubule plus-end binding protein EB3-GFP. EB3-GFP localizes along the leading process with an increasing concentration toward the tip. These cells show random extension of leading process. Fig. 3F is made from this movie.
Movie 7. Dynamics of leading processes on N17-Rac-expressing cells visualized with co-expressed EGFP. These cells show a severe defect in leading tip extension. Fig. 4A is derived from this movie.
Movie 8. Dynamics of terminal structure on the leading tip of neuronal precursors overexpressing Rac visualized with co-expressed EGFP. This structure displays multiple protrusive spikes that turnover rapidly. Fig. 4B is made from this movie.
Movie 9. Dynamics of Δp85α-expressing neuronal precursors visualized with co-expressed EGFP. These cells do not form long leading process and show active random migration. Fig. 5A originated from this movie.
Movie 10. Dynamics of KD-p110γ-expressing neuronal precursors visualized with co-expressed EGFP. These cells do not show any defect in directed extension of leading process. Fig. 5B is derived from this movie.
Movie 11. Dynamics of leading protrusions on C124A-PTEN-expressing cells visualized with co-expressed EGFP. Inhibition of PTEN function enlarges the terminal protrusions. Fig. 5D is made from this movie.
Movie 12. Dynamics of KD-PAK-expressing neuronal precursors visualized with co-expressed EGFP. These cells show the rapid formation and disappearance of branched protrusions at the tip of the leading process. Fig. 6A is derived from this movie.
Movie 13. Dynamics of PAK-overexpressing cells visualized with co-expressed EGFP. These cells display a large lamellar structure at the terminus of their leading process. Fig. 6B is made from this movie.
Movie 14. Dynamics of EGFP-expressing neuronal precursors treated with 100 μM blebbistatin, a myosin II ATPase inhibitor, for 15 minutes. Inhibition of actomyosin contraction reduced spreading of leading protrusions. Fig. 6C originated from this movie.
Movie 15. Dynamics of C3 toxin-expressing neuronal precursors visualized with co-expressed EGFP. These cells show reduced spreading and/or shrinking of leading protrusions. Expression of C3 toxin also affects the direction of terminal protrusions. Fig. 6D is derived from this movie.
Movie 16. Dynamics of ErbB4ΔIC-expressing neuronal precursors visualized with co-expressed EGFP. These cells are showing severe defect in their leading tip extension. Fig. 7B is made from this movie.
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