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Figure 4


Fig. 4. Rac is required for generation of the protrusion on the tip of the leading process. (A) Dominant-negative mutant of Rac (N17-Rac) was co-expressed with EGFP in cerebellar rhombic-lip-derived neuronal precursors. EGFP fluorescent images were captured every 2 minutes for 2 hours (see supplementary material Movie 7), and images at 0, 40, 80, 120 minutes are shown. N17-Rac suppressed the formation of the protrusion on the TLPs and inhibited their extension. (B) Overexpression of Rac induced the formation of multiple protrusive structures at the TLP, whose extension was inhibited (see supplementary material Movie 8). Bar, 10 µm.





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