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Fig. 5. Reduced p63 expression in the bi-transgenics. (a) Immunohistochemical staining of a dorsal skin section from E18.5 wild-type and K5tTA/TRE-KLF5 bi-transgenic embryos with anti-p63 (4A4) antibody. Bar, 50 µm. (b) RT-PCR analysis of different transcripts from skin of E16.5 and E18.5 embryos. Lane 1, E16.5 wild type; lane 2, E16.5 bi-transgenic; lane 3, E18.5 wild type; lane 4, E18.5 bi-transgenic.