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Figure 3


Fig. 3. Dominant-negative effect of R664X AE1 on functional expression of wild-type AE1 in Xenopus oocytes. (A) 36Cl--uptake of oocytes injected with increasing amount of wild-type ({circ}) or R664X (bullet) AE1 RNA. (B) 36Cl--uptake into oocytes injected with wild-type AE1 RNA (WT) with or without various amounts of R664X RNA (R664X). Values indicate DIDS-sensitive 36Cl--uptake calculated by subtracting the mean values in the presence of 10 µM DIDS (n=5-10) from values obtained in the absence of DIDS. Data are expressed as mean ± s.d. (n>=10). *P<0.05, **P<0.01 in oocytes injected with 0.5 ng or 1 ng/oocyte of wild-type RNA alone.





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