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Figure 5


Fig. 5. Intracellular localization of wild-type and R664X AE1 in HEK293 cells. HEK293 cells were transiently co-transfected with the EGFP-wild-type (EGFP-WT) or EGFP-R664X (EGFP-R664X) AE1 and GPC (EGFP-WT/GPC or EGFP-RX/GPC) and stained for GPC with the anti-GPC antibody. HEK293 cells expressing EGFP-wild-type or EGFP-R664X AE1 (EGFP-WT or EGFP-RX) were also incubated with anti-calnexin (Calnexin), anti-GM130 (GM130), and anti-Lamp2 (Lamp2) followed by detection with a secondary antibody labeled with Alexa-Fluor-568. Bars, 10 µm.





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