spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 1


Fig. 1. Nedd4 binds to Cx43 in vitro. (A) Schematic presentation of Cx43 and the GST-fusion proteins used. The four Cx43 transmembrane domains (TM) are shown as black boxes. (B) SDS-PAGE and Coomassie staining of proteins obtained in GST pull-down assays from WB-F344 cell lysates. In pull-down experiments using GST-fusion protein Cx43 CTer, the tight-junction-associated proteins zonula occludens-1 (ZO-1), zonula occludens-2 (ZO-2), and the ubiquitin-protein ligase Nedd4 were identified after nanoESI-MS. (C) SDS-PAGE and immunoblot with an anti-Nedd4 antibody after GST pull-downs from WB-F344 cell lysates.





Right arrow Return to article