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Figure 4


Fig. 4. Peptides containing the PY motif of the Cx43 C-terminus bind to rNedd4 WW2. (A) Peptide sequences of the Cx43 N-terminus (Cx43 NT) and C-terminus (Cx43 CTphosph and Cx43 CT) used in the SPR. All three peptides were N-terminally biotinylated (bio-). The putative PY motif is indicated in red. Phosphorylated serine residues within the peptide are shown as pS. (B-D) BIAcore response curves produced by injecting increasing concentrations of isolated GST-WW2 fusion proteins over the immobilized peptides Cx43 NT (B), Cx43 CTphosph (C) and Cx43 CT (D), respectively. The concentrations of analytes were 0.048 µM (pink), 0.095 µM (light green), 0.189 µM (turquoise), 0.380 µM (blue), 0.761 µM (purple) and 3.043 µM (green). SPR response values are expressed in resonance units (RU).





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