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Figure 5


Fig. 5. The effects of BFA on the vimentin cytoskeleton are reversible. SW13 v+ cells were either untreated (Control) or treated with 10 µg/ml BFA for 30 minutes at 37°C. Cells were then washed and allowed to recover in HEPES-buffered DMEM for 0, 5, 15 or 30 minutes. Following recovery, cells were processed for immunofluorescence and stained with monoclonal antibodies directed against vimentin. Vimentin staining was visualized by epifluorescence microscopy. Changes in the morphology of the vimentin cytoskeleton were fully reversible 30 minutes following drug treatment. Bar, 20 µm.





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