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Figure 6


Fig. 6. Vimentin cytoskeletal architecture is modified by the ARF1 dominant-negative mutant T31N. SW13 v+ cells were transiently transfected with either wild-type ARF1, ARF1-T31N or ARF1-Q71L fused to GFP. Cells were then fixed and processed for immunofluorescence and stained with antibodies directed against either vimentin (a) or the {delta} subunit of AP-3 (b). (a) ARF1-T31N induced process formation in vimentin networks similar to BFA-treated cells. Approximately 66% of cells expressing ARF1-T31N showed either process formation or retraction (quantification on right). The wild type and Q71L mutants did not show the same effects as T31N on vimentin networks. (b) ARF1-T31N, but not the wild type or Q71L mutants, induced release of the adaptor AP-3 from the membrane. Bars, 10 µm.





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