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Figure 3


Fig. 3. CI in N. longicornis. (A) Category 1. In 13% of N. longicornis CI embryos, normal segregation is observed. (B) Category 2. In 2% of N. longicornis CI embryos, the paternal genome is not segregated (arrow). (C,D) Category 3. In 21% of N. longicornis CI embryos, the paternal genome segregates to one daughter. (C) Nuclear cycle 2, one normal nucleus and one composite nucleus (arrowhead). (D) Nuclear cycle 3, two normal nuclei and one composite nucleus (arrowhead) that attempts mitosis. (E-G) Category 4. In 65% of N. longicornis CI embryos, the paternal genome mis-segregates to both daughter nuclei. (E) Nuclear cycle 2, the nuclei are tear-shaped indicating aneuploidy. (F) Nuclear cycle 3, all four nuclei are connected by chromatin bridges. (G) The embryo is composed of a single, diffuse mass of DNA. (H,I) Control uninfected embryos. (H) Nuclear cycle 3, all nuclear are uniform in size and shape. (I) A small number of control embryos contain an odd number of nuclei. Bars, 8 µm.





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