|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 4. Suppression of Aurora B kinase activity compromises cell proliferation and viability. (A) Line graphs plotting relative cell number over an 8-day time course in the continuous presence of tetracycline, showing that like exposure to ZM1, induction of the Aurora B kinase mutants inhibits cell proliferation. Results are from a representative experiment in which each value represents the mean of three assay wells. (B,C) Transgenic lines were induced with tetracycline for 24 hours, replated in the absence of tetracycline, then fixed 17 days later and stained with crystal violet to determine colony number. Images of culture plates (B) and bar graph quantifying cell number (C), both showing that the Aurora B D-N mutant mimics the effect of ZM1. The data are derived from a representative experiment in which each value represents the mean of two assay plates.
|
