spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. Aurora A kinase activity is required for spindle bipolarity. Transgenic lines encoding RNAi-resistant Aurora A transgenes were transfected with siRNAs designed to repress Aurora A or Lamin B1, then exposed to tetracycline as indicated, to induce transgene expression. (A) Immunoblot showing simultaneous repression of endogenous Aurora A and induction of Myc-tagged Aurora A D-N. The arrow indicates the Myc-tagged exogenous Aurora A, whereas the asterisk indicates endogenous protein. (B) Immunofluorescence images showing monopolar spindles in Aurora A RNAi cells expressing the Aurora A transgenes. In panels i and iii, the horizontal arrows indicate bipolar Aurora-A-positive spindles in untransfected cells and the arrowheads indicate prometaphase-like Aurora-A-deficient cells. In panels ii and iv, the vertical arrows indicate bipolar or monopolar spindles, respectively, in cells expressing the Aurora A transgene. Bars, 10 µm. (C) Bar graph quantifying monopolar spindles showing that although the wild-type Aurora A rescues the RNAi phenotype, the Aurora A D-N kinase mutant does not. The values represent the mean ± s.e.m. derived from three independent experiments in which at least 100 mitotic cells were scored.





Right arrow Return to article