JCS03090 Supplementary Material

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• Supplemental Figure 1 (Adobe PDF) -

  Fig. S1. Fluorescence recovery after photo bleaching (FRAP) analysis of GFP-tagged T199E, T219E and T234/237E phosphorylation mimicking mutants. Cells were transfected with GFP-expressing construct and subjected to photobleaching 24-36 hours post transfection. Five scans were taken before bleaching. An area in the nucleolus or a whole nucleolus was bleached with the 488 nm laser and the fluorescence recovery of the bleached spot was measured every 5 seconds. The normalized fluorescence recovery in the region of interest (ROI) at each time point after photobleaching was plotted against time. The t₁/₂s of recovery for the mutants are indicated in the figure. Error indicates s.d. of the mean for each fitted curve.