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Figure 1


Fig. 1. p65 binds to 14-3-3 proteins in response to TNF{alpha}. (a) Pull-down experiment using GST-14-3-3{eta} and lysates from untransfected HEK-293T cells incubated with TNF{alpha} at the indicated times. Upper panel shows immunoblot with anti-p65 antibody. Ponceau staining of GST proteins is shown in the lower panel. Inputs represent 1/10 of the lysates. (b) Cell lysates from HEK-293T, untreated or treated with TNF{alpha}, were precipitated with anti-p65 antibody or rabbit IgG as a control. The presence of 14-3-3 in the precipitates was determined by immunoblotting with anti-14-3-3ß antibody recognizing different 14-3-3 isoforms ({alpha}-14-3-3). Inputs represent 1/10 of the lysates. (c) Schematic overview of the p65 protein sequence indicating the putative 14-3-3 binding domains.





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