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Figure 10


Fig. 10. Effects of Ins(1,4,5)P3-uncaging on Ca2+ oscillations over large areas; anterior (left) and posterior cell poles (right). Recordings of Ca2+ signals (red) before and after the release of caged Ins(1,4,5)P3 by UV light compared to the contraction period (black) of the contractile vacuole. Ca2+ signals were recorded on the anterior or posterior part of the same cell, away from contraction sites. Peaks of black curves correspond to the contracted state of the vacuole. During baseline recordings spontaneous Ca2+ oscillations are observed. Illumination with UV light (gray bar) for uncaging of Ins(1,4,5)P3 does not allow to recognize a global increase of Ca2+ signals, but affects the periodicity of the oscillating Ca2+-signal.





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