spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Characterization of polyclonal antibodies against IP3RN. (A) Affinity-purified anti-IP3RN Abs recognize the polypeptide corresponding to IP3RN residues R896-Q1001 (AG) with high affinity in immuno-blots (second and third lanes), whereas the preimmunserum (PIS) does not show any interaction (fourth lane). The first lane, C, shows 2 µg of the purified AG (Coomassie Blue-stained) used for immunization. (B) Western blot analysis using anti-IP3RN Abs. 100,000-g pellet of whole Paramecium cell homogenate (left lane) was extracted with 2% Triton X-100 and insoluble proteins (middle lane) were separated from soluble proteins (right lane). (C) Subcellular distribution of IP3RN in Paramecium cells. Immunofluorescence analysis shows that Abs against IP3RN stain the ORS.





Right arrow Return to article