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Figure 6


Fig. 6. Influence of extracellular [Ca2+]o on IP3RN gene expression. (A) Immuno-fluorescence images using IP3RN-specific Abs (upper panels, {alpha}-IP3RN) or Abs against V-type H+-ATPase (lower panels, {alpha}-vATPase). Cells were exposed for 24 hours to different levels of [Ca2+]o as indicated. IP3RN seems to be downregulated with decreasing [Ca2+]o. Images were acquired and processed under strictly identical conditions. (B) RNA prepared from cells, which were incubated for 24 hours with different [Ca2+]o, was analyzed by RT-PCR using primers against PtIP3RN or against Ptactin8 (control). (C) Quantification of fluorescence confirms downregulation of IP3RN mRNA compared with actin-8.





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