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Fig. 8. Recordings of Ca2+ signals in close proximity to the ORS. (A) Elevated by a large Ca2+ signal traveling through the whole cell, small Ca2+ sparks localized close to the ampullae and radial arms (RA) can be observed (arrows) These spontaneous subcellular Ca2+ signals move along the ORS (CV, contractile vacuole). (B) Line tracings of the different spots marked by colored arrows in A. Trace d represents the large Ca2+ signal which elevates the small additional Ca2+ sparks visualized in traces a-c.