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First published online September 7, 2006

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Transcription sites: pair and pair alike

Transcription by RNA polymerase II occurs in discrete transcription sites scattered throughout the nucleus. Recent work indicates that coordinately regulated genes are recruited to the same transcription sites, but what controls their recruitment to these sites? Alexandra Binnie, Nicholas Proudfoot and co-authors have used the transcription domains (TDs) formed when transiently transfected plasmids are transcribed as a model system to study this process (see p. 3876). By co-transfecting plasmid pairs into HeLa cells, they show that plasmids containing homologous transcribed sequences form shared TDs. By contrast, those containing non-homologous transcribed sequences enter separate TDs, even if they have homologous backbone or promoter sequences. They also find that a low copy number promotes formation of TDs, but high concentrations of one plasmid do not inhibit formation of TDs by a non-homologous, low-copy-number plasmid. The authors therefore propose that homology between transcribed sequences drives recruitment of genes into TDs; a similar mechanism might recruit coordinately regulated endogenous genes to shared transcription sites.

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Related articles in JCS:

Homologous gene sequences mediate transcription-domain formation

Alexandra Binnie, Pedro Castelo-Branco, Joan Monks, and Nicholas J. Proudfoot
JCS 2006 119: 3876-3887. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content Social Bookmarking                         What's this?