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Figure 1


Fig. 1. Endogenous GBF1 localizes to ß-COP positive peripheral VTCs at steady state. (A) Images a and b. NRK cells were fixed and processed for confocal IF using affinity-purified antibodies against GBF1 [9D5 (a) or 9D2 (b)]. Arrows mark peripheral 9D2-positive puncta. Bar, 5 µm. Images c-e. NRK cells were fixed and processed for standard IF in the presence of 1 µg GBF1 antigen using monoclonal anti-ß-COP antibody and either 9D5 (c,d) or 9D2 (e,f). Bar, 10 µm. (B) NRK cells were fixed and processed for standard IF using affinity purified 9D2 and ß-COP antibody. Bar, 5 µm.





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