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Figure 5


Fig. 5. BFA treatment traps GBF1 onto membranes. (A) NRK cells expressing GFP-GBF1 were incubated on ice for 15 minutes with 5 µg ml-1 NOZ prior to transfer onto microscope stage (37°C). Following equilibration, BFA (5 µg ml-1) was added and FRAP performed as for 3B. Bar, 5 µm. (B) FRAP was performed as for 3A except that cells were treated with 5 µg ml-1 BFA for 10 minutes prior to bleach. ROI size was similar to that used for untreated cells in Fig. 3A. Bar, 5 µm. (C) Quantification of panels 5A (+BFA) and 3D (-BFA) showing relative Golgi/total ratio plotted against treatment length. (D) Quantification of panels 5B (+BFA) and 3A (-BFA) showing relative Golgi/total ratio plotted against treatment length.





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