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Figure 8


Fig. 8. GBF1 decorates matured VTCs labeled by VSVGtsO45. COS-1 cells infected with VSV tsO45 were incubated at 40.5°C for 3 hours (0 min) (a-c) and then shifted to permissive temperature 32°C for either 1 min (d-i) or 6 minutes (j-l). Cells were fixed and then processed for standard IF using monoclonal antibodies against VSVG (a,d,g,j) and polyclonal antibodies against either COPII (c,f) or GBF1 (i,l). Middle panels (b,e,h,k) show merged left and right images. Bar, 5 µm.





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