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Figure 9


Fig. 9. Microinjection of anti-GBF1 antibodies specifically causes membrane dissociation of the COPI but not the clathrin coat. HeLa cells were microinjected with affinity-purified polyclonal anti-GBF1 antibodies (9D2), and fixed 2 hours post-injection. Microinjected cells, identified using goat anti-rabbit antibody, are indicated by white stars. COPI or clathrin coats were revealed using anti-ß-COP (M3A5) or anti-clathrin (X22) monoclonal antibodies. Bars, 10 µm.





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