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Figure 1


Fig. 1. Alterations in nucleolar structure of ask1-1 plants are observed in intact male meiocytes. (a) Prophase I spreads of meiotic chromosomes in wild-type (A-D) and ask1-1 (E-H) cells prepared using Carnoy fixation with enzymatic digestion and acid clearing. (A,E) interphase; (B,F) leptotene; (C,G) zygotene; (D,H) pachytene. Alterations in the association of chromatin with the nucleolus and the nuclear membrane are not observed. (b) Meiotic chromosome analysis in wild-type (A-D and I-L) and ask1-1 (E-H and M-P) cells fixed in parafomaldehyde and lightly squashed without additional treatment. (A,E) interphase; (B,C,F,G) leptotene; (D,H,I,M) zygotene; (J,N) pachytene; (K,L,O,P) diakinesis. Large arrows indicate the position of the nucleolus and nucleolus-associated chromatin in ask1-1. Small arrow points to the attachment of chromatin to the nuclear envelope in ask1-1. Meiotic stage assignment for ask1-1 meiocytes is based on chromatin structure and the stage of the surrounding tapetal cells (Wang et al., 2004). Bars, 10 µm.





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