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Fig. 7. Golgi disruption caused by treatment with PKA inhibitors. (A) Non-transfected cells were incubated or not (-) with 20 µM of either H89 or myristoyl-PKI (mPKI) for 2 hours at 37°C. They were fixed and processed for immunofluorescence with anti-giantin antibody. (B) The average number (± s.e.m.) of Golgi fragments per cell (n=25) was determined from non-transfected cells incubated for 2 hours at 37°C with the indicated concentrations of each inhibitor and stained for immunofluorescence with anti-giantin antibody. (C) Golgi-associated fluorescence was determined from cells (n=20) incubated or not (-) for 2 hours at 37°C with 20 µM of each inhibitor. (D) Cells stably expressing GalTfase-CFP were similarly incubated, fixed and processed for immunofluorescence with anti-giantin antibody. Bars, 10 µm.
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