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Figure 1


Fig. 1. Syntaxin 1A facilitates EAAC1 endocytic sorting. (A) Representative immunoblot and quantitative analysis showing that in C6 glioma cells transfected with syntaxin 1A (Syn1A), the expression of endogenous EAAC1 is decreased in both total lysate and cell surface fractions. Actin served as an internal control for protein loading. Data are plotted as a percentage of EAAC1 in the cells transfected with ß-gal within each fraction. **P<0.01 compared with cells transfected with ß-gal (n=3). (B) Representative immunoblot and quantitative analysis show that the delivery efficiency of EAAC1 is not altered in Syn1A-transfected C6 glioma cells. The data are plotted as a percentage of the increased cell surface fraction versus the intracellular EAAC1 pool available for surface delivery (n=3). (C) Representative immunoblot and quantitative analysis of internalized EAAC1 show that the internalization of EAAC1 is potentiated in Syn1A-transfected C6 glioma cells (upper panel), despite syntaxin 1A decreasing the overall EAAC1 surface pool available for internalization (lower panel). The band intensities for the internalized EAAC1 are plotted as a percentage of the total surface EAAC1 available for internalization within each transfection group. **P<0.01 compared with the cells transfected with ß-gal at the same time point (n=3).





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