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Figure 3


Fig. 3. Insulin increases Akt activity but does not reverse TNF-induced apoptosis or reduce MK2 activity in Adv-infected cells. Adv- and IFN{gamma}-sensitized HT-29 cells were treated with TNF (100 ng/ml and 50 ng/ml, respectively) in the presence and absence of (A-C) 100 nM insulin or (D-F) 20 µM LY294002. Cells were lysed at 12 hours and assayed for kinase activity, or collected after 24 hours and assayed for apoptosis by flow cytometry for cleaved caspase-cytokeratin. (A,D) Change in TNF-induced cell death. Measurements are plotted as the mean of six biological replicates (two independent experiments) ± s.e.m. (B,E) change in TNF-induced Akt activity. Measurements are plotted as the mean of six biological replicates (two independent experiments) ± s.e.m. (C,F) Change in TNF-induced MK2 activity in Adv-sensitized cells. Measurements are plotted as the mean of three biological replicates ± s.e.m. Changes are labeled as significant (*) if P<0.05.





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