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Figure 4


Fig. 4. Adv infection saturates phosphorylation of downstream Akt effectors. (A) Cells were treated as described in Fig. 3, lysed at 12 hours and analyzed by western blot by probing with anti-pGSK-3{alpha}/ß and anti-Akt-pSub. Experimental conditions are the same as those described in Fig. 3. 10% fetal bovine serum (FBS) stimulation for 15 minutes was used as the positive-control stimulus. 50 µg protein was loaded into each lane. (B,C) Adv-sensitized and IFN{gamma}-sensitized (B) pGSK-3{alpha} and (C) Akt-pSub42kDa bands were quantified by densitometry and normalized to the sensitized (Adv or IFN{gamma}) 0-min band. Measurements are plotted as the mean of three biological replicates ± s.e.m. Changes are labeled as significant (*) if P<0.05.





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