QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 4. Helix-1 contains a distinct motif that facilitates Golgi targeting. (A) Cos1 cells were transfected with a construct in which the Asp5 in (1-25)-4A1-GFP was mutated to Ala [D5A(1-25)-4A1-GFP, green]. Cells were then fixed and co-stained with the Golgi marker, BODIPY ceramide, the ER probes, ERtracker, the lysosomal probe, Lysotracker, the mitochondrial probe, Mitotracker or the Early endosome marker, EEA1 (all red). Merged images are shown in final column. (B) Mean percentage (± s.e.m.) co-localisation between D5A(1-25)-4A1-GFP and the various organelle probes used (n=25 cells). (C) Cells were transfected with the constructs L3AV4A(1-25)-4A1-GFP and F5AF6A(1-25)-4A1-GFP (green), co-stained with BODIPY ceramide (red) and then visualised live. Merged images are shown where indicated. (D) Cells were transfected with the constructs L3A(1-25)-4A1-GFP, V4A(1-25)-4A1-GFP, F5A(1-25)-4A1-GFP or F6A(1-25)-4A1-GFP (green), probed with the Golgi marker BODIPY ceramide (red) and then visualised live. Merged images only are shown. Co-localisation is indicated in yellow. Bars, 10 µm. (E) Percentage co-localisation (± s.e.m.) of the constructs described in D with the Golgi probe BODIPY ceramide (n=25 cells).

Return to article