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Fig. 3. Specificity and potency of the BMP receptor-II ectodomain at neutralising oocyte- and GDF9-stimulated mural GC DNA synthesis. (A) Mural GCs were cultured with DOs (12 DOs/125-µl well), mouse GDF9 (40 ng/ml), human activin A (20 ng/ml), and human TGFß1 (0.5 ng/ml), in either the absence or the presence of a maximum neutralising dose of BMP receptor-II ectodomain (BMPR-II ECD; 2 µg/ml). Bars represent means ± s.e.m. from at least triplicate wells and are representative of three replicate experiments. Asterisks represent significant neutralisation (P<0.01) caused by the ECD. Control is mural GC alone or with mitogen. (B) Mural GCs were cultured either with or without DOs (12 DOs/125-µl well; 
) or 40 ng/ml mouse GDF9 (
), in either the absence or the presence of increasing doses of BMPR-II ECD. Points are means ± s.e.m. from at least triplicate wells, expressed as a fraction of the control (mitogen with no ECD), and are representative of three replicate experiments. An asterisk represents the lowest dose of BMPR-II ECD that is significantly less than the 100% control (P<0.001).
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