spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 7


Fig. 7. Processing of laminin {alpha}2 subunit by MT1-MMP. (A) Schematic representation of the heterotrimeric laminin-2, the localization of two epitopes of the antiserum and the corresponding regions of three processed fragments `a', `b' and `c' appeared in (B) and (C). Open arrow indicates a putative cutting site by MT1-MMP that generates the band `b'. Closed arrow indicates additional cleavage site that is available only in biochemical assay (discussed in Results). (B) Western blot analysis of muscle tissues obtained from 4-week-old wild type (WT) and MT1-MMP-deficient mice (MT1-MMP-/-) using anti-300K antiserum. Three arrowheads in the right indicate the major fragments detected. (C) The purified laminin-2 from human placenta was digested with the recombinant catalytic fragment of MT1-MMP (1:100, 1:50 molecular ratio in w/w) at 37°C for 18 hours. The resulting products were analyzed by western blotting, using either anti-300K or anti-IVa. Three arrowheads in the right indicate the corresponding fragments of those detected in (B).





Right arrow Return to article