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Fig. 1. Microscopy of live vegetative cells expressing GFP-ABD. Fluorescent images of GFP-ABD-expressing, vegetative wild-type (A-D) and myosin-II-null (E) cells in SB were captured sequentially with a CCD camera. (A and B are two excerpts from the same sequence. See supplementary material Movie 1.) Intercalated phase-contrast images are shown at t=20 seconds in A, 0 seconds in B, and 2 seconds in E. In A, every frame is shown from left to right. The arrowhead indicates a spherical bleb that formed suddenly; it was initially devoid of F-actin cortex but gradually acquired it. In B, every second frame is shown sequentially from left to right. (C) Higher magnification of the blebbing domain followed in B. The arrow indicates examples of blebs forming beside pre-existing or growing filopodia. The asterisk shows a detached cortical layer moving centripetally. (D) Kymograph obtained by scanning in the direction of the dotted arrow shown in C for the corresponding period. The vertical ticks on the time axis (t) are separated by 1 second. The arrow, arrowhead, and asterisk correspond to F-actin layers shown in B and C. (E) Every second frame is shown from left to right. Bars, 10 µm (A-C,E); 3 µm (D).
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