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Figure 6


Fig. 6. Effects of buffer osmolarity on velocity of cell movement. The mean values of the rates of gain (A) and loss (B) of cell area, and the centroid velocities (C) are plotted as a function of the sorbitol concentration. Filled symbols, wild type; open symbols, myosin-II-null cells. Data from a slow strain of myosin-II-null cells are shown. Data of various lengths, from 43-149 cells for filled circles and 18-60 for open circles are shown. The error bars indicate the s.e.m. (D) Mean values of the rates of gain-of-area measured in buffers without (SB) or with (HI) 100 mM sorbitol, measured 30-60 minutes after application of 100 µM of blebbistatin (BLEB) or 0.2% DMSO (DMSO). WT and MII-indicate wild type and a fast strain of myosin-II-null cells, respectively. Data were normalized to the mean areas of the corresponding cell and then pooled for statistics (n=22-38 cells).





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