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Fig. 2. TGF-ß is upregulated and latent TGF-ß complexes are degraded in the morpholgically altered epilysin-expressing A549 cells. (A) Conditioned serum-free media were harvested from A549 cell pools, and the concentrations of TGF-ß in the untreated (active TGF-ß) or heat-treated (total TGF-ß) media were determined using indicator cells. The results are shown as relative TGF-ß activity where the activity in the medium from the control pool has been set to 1. The asterisk denotes statistically significant difference in the total TGF-ß levels over the control (determined using the Student's t-test, P<0.05). (B,C) Polypeptides in the conditioned media from the cell pools were immunodetected with antibodies specific for latent complexes containing TGF-ß1 (anti-TGF-ß1-LAP) and LTBP-1 as indicated. In panel B the intact large latent TGF-ß1 complexes in the Ctrl and Epi-E/A samples (filled arrow) and proteolytically processed complexes in the Epi sample (unfilled arrow, band encircled) have been indicated. In panel C the intact TGF-ß/LTBP-1 complexes have been indicated with a filled arrow, and the proteolytically processed, truncated forms of LTBP-1 with a bracket.
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