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Figure 3


Fig. 3. Epilysin-induced EMT is prevented by the MMP-inhibitor GM6001 and by anti-TGF-ß antibodies. (A) Epilysin-expressing A549 pools were selected in the presence of GM6001 (1 µM), which prevented the epilysin-induced EMT. The cells were seeded on glass coverslips in 10% serum containing medium with the indicated supplements. After 48 hours the cells were changed to serum free medium containing the same supplements. After 24 hours the amounts of total and active secreted TGF-ß in the media were determined. The TGF-ß levels in the control and E/A pools are shown on the right. (B) Living cells were incubated with anti-E-cadherin antibodies, fixed with PFA, permeabilized and then stained with antibodies against the phosphorylated form of SMAD2. (C) The numbers of E-cadherin and P-SMAD2 positive cells in each culture were counted. The proportions of positive cells in each culture are given.





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