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Fig. 7. Epilysin increases the migration of A549 cells. Cell culture plate inserts with 8 µm pore membranes were coated with type I collagen (0.1 mg/ml). Stable A549 cell pools were added to the upper chamber. The MMP-inhibitor GM6001 (10 µM) was then added to both chambers as indicated. After 4 hours cells that had migrated through the membrane were stained and counted. The asterisk denotes statistically significant variation between indicated samples (Student's t-test, P<0.05).