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Figure 6


Fig. 6. CDK7 and XPB co-immunoprecipitations (CoIP) of cytoplasmic and nuclear fractions from embryos at mitotic stages 8-14. The cytoplasmic fraction was immunoprecipitated with either an anti-CDK7 or anti-XPB antibody. The nuclear fraction was co-immunoprecipitated with the anti-CDK7 antibody. The precipitated material and total proteins from cytoplasmic fraction before CoIP were analyzed by SDS-PAGE. The proteins were transferred to a membrane and analyzed by western blot experiments for the presence of CDK7, MAT1, XPB and XPD using specific antibodies as indicated. The same blot was reused for each antibody. The antibody used for CoIP is indicated in the figure. Lanes labeled P show the CoIP material and those labeled I show the input from the original cytoplasmic or nuclear fraction, equal amounts of protein were loaded in each lane.





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