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Figure 3


Fig. 3. Plasmid TDs localise to the interchromatin compartment, whereas punctate plasmid transcription sites overlie the cellular chromatin. (A) Plasmid pHIV-ß was transiently transfected into HeLa cells expressing a GFP-histone H2B construct (second panel). Plasmid transcription was labelled by RNA in situ hybridisation using probes to the ß-globin first intron (first panel). Plasmid TDs localised to the interchromatin compartment as shown by the lack of red/green colocalisation in the overlay image (third panel). (B) Punctate plasmid transcription signals (first panel) showed significant overlap with the H2B histone staining (second panel) as shown by the overlay image (third panel). Bars, 5 µm.





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