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Fig. 4. Homologous gene sequences mediate TD formation. (A) HeLa cells were transiently transfected with plasmids pHIV-ß and pHIV- and the plasmid transcription sites were labelled by RNA in situ hybridisation with probes to the ß-globin (first panel) and 2-globin (second panel) first introns. The plasmids formed separate TDs as shown by the absence of red-green colocalisation in the overlay image (third panel). Bar, 1 µm. (B) Line scans showing the relationship between the pHIV-ß (green) and pHIV- (red) transcription signals. The scans are 2D intensity plots corresponding to the yellow lines in Fig. 2A and are labelled as (i) and (ii) accordingly. There is minimal correlation between the pHIV-ß and pHIV- signals and the signal peaks do not overlap. (C) HeLa cells were transiently transfected with plasmids pHIV-ß and pHIV-ß, which share 1243 bp of homologous ß-globin gene sequence, and the transcription sites were labelled by RNA in situ hybridisation with probes to the ß-globin (first panel) and 2-globin (second panel) first introns. The plasmids formed shared TDs as shown by the yellow signals in the overlay image (third panel). Bar, 5 µm. (D) Line scans showing the relationship between the pHIV-ß (green) and pHIV- (red) transcription signals. The scans are 2D intensity plots corresponding to the yellow lines in Fig. 2C and are labelled as (i) and (ii) accordingly. The plots indicate that there is significant correlation between the pHIV-ß and pHIV-ß signals.

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