spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. mAtg9 is present on late endosomes. (A) Western blots of fractions from an endosome preparation gradient from rat liver using antibodies for EEA1 (early endosomes), mAtg9, CI-MPR, TGN38 (TGN), p58 and PDI (ER). To label late endosomes, HRP-biotin was internalized by perfusion for 10 minutes followed by a 20-minute chase before homogenization. HRP-biotin was detected using ExtraAvidin-HRP. Signals were quantified by densitometry, performed using ImageJ. Data are representative of two independent experiments. (B) mAtg9 colocalizes with the CI-MPR in indirect immunofluorescence on HEK293 cells. Inset is enlargement of the peripheral staining in the merge. Bar, 10 µm. (C) Cryoimmunogold labelling of mAtg9 (red arrowheads, 10-nm gold), CI-MPR (green arrowheads, 15-nm gold), on HEK293 cells labelled with 6-nm conjugated BSA-Gold (arrows) internalized for 2 hours. Bar, 200 nm.





Right arrow Return to article