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Figure 5


Fig. 5. mAtg9 colocalizes with Rab6 on Golgi membranes, and Rab7 and 9 on late endosomes. HEK293 cells were transiently transfected with GFP-Rab6 (A), GFP-Rab7 (B) and GFP-Rab9 (D), then 24 hours later fixed and labelled with the mAtg9 antibody. Cells were also labelled with antibodies to endogenous Rab7 (C) and 9 (E), the inset is an enlargement of the merge to show the peripheral staining. For Rab7 staining, cells were extracted using 0.05% (w/v) saponin before fixation. Bars, 10 µm.





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