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Figure 7


Fig. 7. mAtg9 and CI-MPR redistribute upon starvation in a reversible, PtdIns 3-kinase-dependent manner. HEK293 cells were labelled with antibodies against mAtg9 and CI-MPR (A-D) or TGN46 (E-G). Cells had first been treated with either rapamycin (C) for 2 hours, or for 2 hours in ES medium in the presence (D,F) or absence (B,E) of 65 µM LY294002. (G) Cells had been starved for 2 hours in ES medium, before incubation in FM for 2 hours in the presence of 10 µg/ml cyclohexamide. Note: cyclohexamide was also included in the final 15 minutes in ES medium). Bar, 10 µm.





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