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Figure 8


Fig. 8. The starvation-induced dispersal of mAtg9 can be blocked by knockdown of ULK1 but not ULK2. HEK293 cells were treated with either control siRNA (A,B,E,F) or siRNA to either ULK1 (C,G) or ULK2 (D) and incubated in FM (A,E) or for 2 hours in ES medium (B-D,F,G). The cells were then fixed and labelled with antibodies against mAtg9 and TGN46 or CI-MPR. The starvation-induced dispersal of both mAtg9 (B,F) and CI-MPR (F) could be blocked by siRNA-mediated knockdown of ULK1 (C,G) but not ULK2 (D). Bars, 5 µm.





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