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First published online September 20, 2006

Journal of Cell Science 119, 1903e (2006)
© The Company of Biologists Limited
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In this issue

How cells drink


Figure 1

Macropinocytosis - the engulfment of extracellular fluid by cells - is an important but poorly understood physiological process previously studied only in fixed cells. Now, Rohan Teasdale and co-authors have visualized macropinosome maturation in living cells, using time-lapse videomicroscopy (see p. 3967). They report that sorting nexin SNX5, one of a family of hydrophilic proteins that are recruited to the endosomal system by binding to 3-phosphoinositides, is recruited to newly formed macropinosomes when cells are stimulated with epidermal growth factor. Experiments with GFP-SNX5 indicate that it is sequestered into discrete subdomains of the nascent macropinosome and then incorporated into dynamic tubular structures that quickly leave the macropinosome to fuse with early endosomes. The authors show that the early endosomal protein Rab5, a small GTPase, is also recruited by newly formed macropinosomes, followed by Rab7 as macropinosomes mature. In addition, they demonstrate that SNX5 must form dimers with SNX1 before it can associate with macropinosomes. SNX5-positive tubules, the authors suggest, may provide a rapid way to recycle components from early macropinosomes, thereby promoting their maturation.


Related articles in JCS:

Visualisation of macropinosome maturation by the recruitment of sorting nexins
Markus C. Kerr, Margaret R. Lindsay, Robert Luetterforst, Nicholas Hamilton, Fiona Simpson, Robert G. Parton, Paul A. Gleeson, and Rohan D. Teasdale
JCS 2006 119: 3967-3980. [Abstract] [Full Text]  




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