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Figure 1


Fig. 1. At low-level expression GFP-tagged PIKfyve is predominantly associated with the early endosome. (A-E) HeLa cells were transiently transfected with GFP-PIKfyve and, after 24 hours, cells were fixed and stained for the early endosomal markers EEA1 (A) and SNX1 (B), internalised EGF receptor 10 minutes after EGF addition (C), and the late endosomal markers CD63 (D) and LAMP1 (E). Images are single, 0.1 µm confocal sections and are representative of more than ten imaged cells in each case. Bars, 10 µm. Boxed area in A-E is shown enlarged on the right.





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