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Figure 5


Fig. 5. PIKfyve suppression results in the swelling of both early and late endosomal compartments. (A-E) HeLa cells were transiently transfected with (A) PIKfyve-specific siRNA duplex II or (B-E) a combination of PIKfyve-specific siRNA duplexes II and V; left column, EM images; right column, phase-contrast images. Boxed insets show magnified regions. After 72 hours, cells were fixed and stained for (A) internalised transferrin receptor, 10 minutes after the addition of ligand (TfR), (B) LAMP1, (C) CD63, (D) SNX1 and (E) EEA1. For each condition similar images were observed in three independent experiments in which at least five randomly chosen fields of view were analysed. The arrows in (A) highlight the small vacuoles labelled with the internalised transferrin receptor. Bars, 10 µm (A), 25 µm (B-E).





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